Figure 7.

Administration of MA to WT But Not SF-1 KO Mice Significantly Stimulates ERK Phosphorylation in the VMH

WT and SF-1 KO mice were treated with MA and cells that contained phospho-ERK were quantified in sections of the mediobasal hypothalamus as residing in the dorsomedial (dm), central (c), or lateral (lat) regions of the VMH. A, Representative sections of immunoreactive phospho-ERK in vehicle- and MA-treated WT and CNS-specific SF-1 KO mice. B, The ERK phosphorylation induced by MA injection was significantly diminished in CNS-specific SF-1 KO mice. Bars represent means ± sem (*, P < 0.01; **, P < 0.05, n = 3, t test).