Table 2.

Effect of PP5 over expression on MCF-7 cell survival following treatment with agents that induce apoptosis in MCF-7 cells.

Treatment:	UVC (30 mJ)	Adriamycin (1.7 μM)	Vinblastine (20 nM)	Taxol (50 nM)	Rapamycin (200 nM)	Serum Starvation	Rapamycin (200 nM) plus Serum Starvation
	Duration of treatment:
	24 hours		48 hours		72 hours
	Percentage of cell survival; treated/control (mean +/− SD):
Cell line:
MCF-7-EGFP	28.2 ± 1.8	42.8 ± 5.9	33.4 ± 0.8	30 ± 0	64.5 ± 6.7	45.9 ± 2.8	40.0 ± 5.4
MCF-7-PP5s	47.3 ± 4.8	60.6 ± 9.9	38.8 ± 0.7	36.5 ± 6.4	59.5 ± 12.8	42.8 ± 10.2	36.4 ± 8.3
p-value	p< 0.003	p< 0.05	p< 0.02	p< 0.5	p< 0.7	p< 0.7	p< 0.7
MCF-7	33.3 ± 3.2	40.5 ± 3.2	31.6 ± 0.9	39.4 ± 3.7	ND	ND	ND
MCF-7-PP5h1	48.8 ± 3.1	49.0 ± 2.8	39.7 ± 0.4	36.8 ± 2.0	ND	ND	ND
p-value	p< 0.004	p< 0.002	p< 0.007	p< 0.3	ND	ND	ND

Replicate plates of the indicated cell lines were produced by plating cells at equal density. After 24 hours, the cells were treated with the agents shown, and at the times indicated the number of cells in duplicate plates was determined by cell counting using a Coulter Counter. The data shown are the mean of 4 experiments +/− SD. P-values were determined by using the Students t-test (unpaired, two-tailed), and each P-value represents a comparison of treated PP5 over-expressing cells to treated control cells (MCF-7PP5s vs MCF-7-EFGP; MCF-7 vs MCF-7-PP5h1). P values of less than 0.05 were regarded as significant and are indicated by bold font.