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PCBP1 binds to ferritin in vivo. Yeast cells expressing ferritin H- and L-chains, PCBP1, or both were lysed and subjected to immunoprecipitation with anti-PCBP1 or anti-ferritin antibody in buffers containing ferrous iron (A and B), no iron (B), or ferrous iron and a ferrous iron chelator (BPS, B). Immune complexes were detected with antibodies against PCBP1 and ferritin. Note that PCBP1 migrates more rapidly in whole lysates than after IP (A).
