Table 1.
Effects of disruption of the gene encoding SR-BI on plasma total cholesterol and apoA-I concentrations, and adrenal gland total cholesterol content in wild-type (srbI+/+), and heterozygous (srbI+/−), and homozygous (srbI−/−) mutant mice.
| srbI genotype | Gender | F1 generation*
|
F2 generation*
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| Plasma total cholesterol
|
Plasma total cholesterol
|
Plasma apoA-I†
|
Adrenal gland total cholesterol
|
||||||
| mg/dl‡§ | % of control | mg/dl‡§ | % of control | mg/dl‡§ | % of control | μg/mg protein‡§ | % of control | ||
| Male | 93 ± 8 (29) | 100 | 99 ± 12 (18) | 100 | — | — | — | — | |
| +/+ | Female | 80 ± 7 (13) | 100 | 94 ± 20 (27) | 100 | — | — | — | — |
| Both | 89 ± 10 (42) | 100 | 96 ± 17 (45) | 100 | 25 ± 3 (10) | 100 | 128 ± 28 (5) | 100 | |
| Male | 126 ± 10 (21)¶ | 135¶ | 137 ± 21 (29)¶ | 138¶ | — | — | — | — | |
| +/− | Female | 112 ± 9 (23)¶ | 140¶ | 118 ± 19 (49)¶ | 126¶ | — | — | — | — |
| Both | 119 ± 12 (44)¶ | 134¶ | 126 ± 22 (78)¶ | 131¶ | 28 ± 2 (12)‖ | 112‖ | 74 ± 18 (6)¶ | 58¶ | |
| Male | — | — | 220 ± 41 (10)¶ | 222¶ | — | — | — | — | |
| −/− | Female | — | — | 209 ± 32 (7)¶ | 222¶ | — | — | — | — |
| Both | — | — | 216 ± 37 (17)¶ | 225¶ | 27 ± 3 (11)‖ | 108‖ | 36 ± 7 (5)¶ | 28¶ | |
F1 generation animals were not fasted. F2 generation animals were not fasted prior to analysis of adrenal gland cholesterol levels but were fasted for 4–8 h prior to analysis of plasma.
Values determined with an Autoanalyzer and human apoA-I standards.
Values represent mean ± SD.
Values in parenthesis represent the numbers of animals analyzed.
Statistically significantly different from wild-type controls, P < 0.005 or better.
Not statistically significantly different from wild-type controls, independently confirmed by quantitative evaluation of immunoblot analysis.