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. 1997 Nov 11;94(23):12616–12621. doi: 10.1073/pnas.94.23.12616

Figure 3.

Figure 3

Schematic outline of the EBNA4 chimeras. Chimeric EBNA4 proteins were generated by in-frame insertion of sequences coding for the 239-amino acid-long Gly-Ala repeat of EBNA1 (flGA) or a 39-amino acid-long polypeptide containing a Gly-Ala 17 mer flanked by unique EBNA1 residues (GA39) in the MscI site [E4-flGA, E4-GA39(M)] or SspI site [E4-GA39(S)] of EBNA4. Insertion of the flGA cassette in the opposite orientation relative to the EBNA4 sequence yielded the E4-PPA chimera that contains a 238-amino acid-long Pro-Ala repeat.