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Ubiquitination of the Gly-Ala containing EBNA4 chimeras. In vitro-translated EBNA4, E4-flGA, and E4-GA39(M) were incubated for 2 hr at 37°C in crude rabbit reticulocyte lysate containing ATP-γS and ubiquitin aldehyde. Control samples were kept on ice or at 37°C under ATP-depleting conditions. One representative experiment out of three is shown in the figure.
