Figure 6.

Influence of Gly-Ala repeat on the in vivo turnover of EBNA1. The in vivo turnover of EBNA1 and E1-ΔGA was monitored in pulse–chase experiments using CV1 cells infected with the corresponding recombinant vaccinia viruses. One representative experiment out of three. (A and B) In vivo-labeled polypeptides were immunoprecipitated using the OT1x mAb and resolved by SDS/PAGE. (C) The percent degradation was calculated from densitometry scans as follows: 1, intensity of the specific band at the indicated time/intensity of the specific band at time 0 × 100. ○, EBNA1; ⧫, E1-ΔGA.