Table 2.
Donor* | Recipient | Percent of transductants with donor phenotypes
|
|
---|---|---|---|
DnaC+ rK−mK− | DnaC+ TetS | ||
NM854 | NM815 (rK+mK+) | 52 | 96 |
NM854 | NM814 (rK−mK+) | 50 | 96 |
NM840 | NM815 (rK+mK+) | 43 | 96 |
NM840 | NM814 (rK−mK+) | 56 | 94 |
NM679 | NM815 (rK+mK+) | 98 (94)† | 96 (90)† |
NM679 | NM814 (rK−mK+) | 100 (95)† | 97 (92)† |
The order of genes on the chromosome is hsdS hsdM hsdR dnaC Tn10. Cotransduction frequencies are based on a minimum of 50 colonies.
See Table 1 for identification of hsd deletion. The hsdSM deletion in NM854 is tagged by a gene conferring resistance to kanamycin. The other deletions were recognized by their restriction and modification phenotype.
Donor strain for data within brackets was without λhsdM+S+ prophage and therefore mK−.