Table 3.
Donor | Recipient | Percent of transductants with donor phenotypes
|
|
---|---|---|---|
DnaC+ HsdSD | DnaC+ TetS | ||
NM515 (rD+mD+) | NM824 (rK−mK+) | 16 | 80 |
NM807 (rD−mD+) | NM824 (rK−mK+) | 12 | 71 |
NM515 (rD+mD+) | NM822 (rK−mK+, HsdC−) | 0* | 71 |
NM807 (rD−mD+) | NM822 (rK−mK+, HsdC−) | 12 | 72 |
NM807 (rD−mD+) | NM846 (rK+mK+, HsdC−) | 1† | 89 |
The order of the genes on the bacterial chromosome is hsdS hsdM hsdR dnaC Tn10. Cotransduction frequencies are based on at least 50 colonies.
0 in 100 transductants.
One rD−mD+ and two rK−mK+ transductants; the former requires entry of the hsdSD gene and the potential for the production of EcoDI before recombination occurs.