Figure 4.

Southern blot of PCR amplification products of the P2Br allele using tissues from a male heterozygous for the strain 71 PrmCre transgene and the P2Bc allele. DNA from 10 different tissues was amplified by using primers and conditions that produced a 350-bp product from the recombined, P2Br allele (see Fig. 1 and Materials and Methods). Each lane contains 10% of the reactions, except for the testis reactions, which were diluted 500- (T5), 250- (T2), and 100- (T1) fold before loading, and a liver reconstruction control (C), which was diluted 1:100 before loading. (Li, liver; Sp, spleen; Pa, pancreas; Th, thymus; Lu, lung; Ki, kidney; He, heart; Mu, skeletal muscle; Br, brain; No, no DNA control).