Figure 3.

Induction of antimicrobial peptide genes in fungi-infected adults. (A) Northern blot analysis of total RNA extracted from wild-type, Tl⁻, and Tl^D mutant female adults. Flies were anesthetized and covered with spores of B. bassiana. Flies were placed at 29°C and collected after different time intervals. (B) Northern blot analysis of total RNA extracted from wild-type female adults after natural infection by various fungi. Flies were placed at 29°C and collected 5 days later. A and B were obtained separately. C, control; d, days; Tl⁻, Tl⁶³²/Tl^1-RXA female adults; Tl^D, unchallenged Tl^10b female adults; S.i. (septic injury) adults that were challenged by pricking with a needle dipped into a mixture of E. coli and M. luteus and collected 6 h later. B.b., B. bassiana; P.f., Paecylomyces fumoroseus; A.f., A. fumigatus; M.a., Metharizium anasiplae.