Table 1.
The effect of IL-10 and its synthetic analogues, IT9302 and IT9403, on the growth/proliferation of murine mast cells (MC/9), when cultured in the presence of the costimulatory factor IL-4.
| Cytokine/oligopeptide | A Total number of cells in culture | B Total number of cells in cultures pretreated with IL-10sR-mAb for 1 hr | (A-B)/A Relative reduction (%) of the number of cells in cultures as result of IL-10 sR pretreatment |
|---|---|---|---|
| IL-4, 20 ng/ml | 47,000 ± 2,000 | ||
| IL-4 + IL-10 | |||
| (2 ng/ml) (2 ng/ml) | 48,000 ±0 | ||
| (20 ng/ml) (20 ng/ml) | 66,000 ± 6,000 | 52,000 ± 4,000 | 21.2 |
| IL-4 + IT9302 | |||
| (20 ng/ml) (2 ng/ml) | 33,000 ± 1,000 | ||
| (20 ng/ml) (20 ng/ml) | 40,000 ± 0 | 31,000 ± 1,000 | 21.9 |
| IL-4 + IT9403 | |||
| (20 ng/ml) (2 ng/ml) | 48,000 ± 1,000 | ||
| (20 ng/ml) (20 ng/ml) | 64,000 ± 8,000 | 31,000 ± 4,000 | 52.1 |
| IL-4 + IT9403 + IT9302 | |||
| (20 ng/ml) (2 ng/ml) (2 ng/ml) | 45,000 ± 1,000 | ||
| (20 ng/ml) (20 ng/ml) (20 ng/ml) | 79,000 ± 25,000 | 36,000 ± 14,000 | 54.3 |
In some of the cultures, the cells were pretreated for 1 h with a monclonal antibody toward IL-10 sR (2 μg/ml).