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. 1997 Dec 23;94(26):14626–14631. doi: 10.1073/pnas.94.26.14626

Figure 2.

Figure 2

Influence of the route of administration on the IgG subclass responses. At 4 weeks post-initial immunization sera were obtained and assayed for Ag-specific IgG1, IgG2a, or IgG2b in an ELISA. Titers were defined as the highest dilution to reach an OD of 0.2 at 450 nm. (A) BALB/c mice were immunized with 100 μg of CIGH-OVA (DNA) or 100 μg of OVA protein (protein) in normal saline. Results are expressed as the mean of the log10 titer ± SEM from five mice in each group immunized with CIGH-OVA or OVA protein. (B) BALB/c mice were immunized with 100 μg of hIg encoding DNA in normal saline i.m. or i.d. Results are expressed as the mean of the log10 titer ± SEM from six mice in each group. (C) BALB/c mice were immunized i.m. with 100 μg of CIGH-HEL (DNA) or 25 μg of HEL protein (protein) in normal saline. Results are expressed as the mean of the log10 titer ± SEM from five mice immunized with HEL protein and from 16 mice immunized with CIGH-HEL.