Table 4.

Effect of preconditioning recipient mice before SCT on engraftment of fully allogeneic HSCs

Preconditioning	H-2 Kb+ cells, %	H-2 Kd+ cells, %
None	3.5  ±  1.2	97.6  ±  1.7
Thymectomy	27.6  ±  12.3	59.7  ±  18.7
G-CSF injection	29.4  ±  10.6	67.5  ±  16.9

Spleen cells of recipient BALB/c mice transplanted with 1.5 × 10⁵ HSCs (LD, lineage-negative, CD71⁻) from 5-FU-treated B6 donors were stained with FITC-conjugated H-2 K^b (donor type) or FITC-conjugated H-2 K^d (recipient type) 4 months post-SCT. Thymectomy was done with when recipients were 3 to 4 weeks old. G-CSF was given s.c. 6 μg/day/recipient for 5 consecutive days before SCT. Each experimental group consisted of at least three mice. Results are mean ± SD.