Table 3.
CCL21 production.
|
CCL21 in DC supernatant [ng]/cell number | |||||||||||||
| 24 h | 48 h | 72 h | |||||||||||
| DC | CCL21-DC | CV-DC | DC | CCL21-DC | CV-DC | DC | CCL21-DC | ||||||
| Cell number: ×106 | Cell number: ×106 | ||||||||||||
| 1 | 1 | 2.5 | 5 | 10 | 1 | 1 | 1 | 2.5 | 5 | 10 | 1 | 1 | 4 |
| n = 16 | n = 16 | n = 9 | n = 6 | n = 2 | n = 6 | n = 12 | n = 10 | n = 7 | n = 7 | n = 2 | n = 6 | n = 4 | n = 4 |
| ng/cell number | ng/cell number | ng/cell number | |||||||||||
| *0.0 | 2.3 ± 1.9 | 3.8 ± 2.8 | 3.5 ± 3.4 | 7.5 ± 2.4 | *0.0 | *0.0 | 3.7 ± 2.4 | 5.2 ± 2.5 | 4.4 ± 2.9 | 8.7 ± 1.1 | *0.0 | *0.0 | 6.7 ± 1.5 |
* Below detection
The capacity of DC to secrete CCL21 was evaluated by ELISA. Mock-transduced DC (DC), CV-DC and CCL21-DC were cultured at the concentrations indicated in 1 ml culture medium. The supernatant was analyzed after 24, 48, and 72 h cultures. The concentration of CCL21 is expressed in ng per cell number. The values represent the average ± SD of different independent experiments, as indicated.