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. 2008 Jul 29;8:58. doi: 10.1186/1472-6750-8-58

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Copyright © 2008 Hillebrecht and Chong; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Analysis of rabbit reticulocyte lysate by size separation and western blotting. Top, SDS-PAGE analysis of the peak fractions (9 to 18) from the size-exclusion separation of a crude rabbit reticulocyte lysate. The molecular weight standards for the SDS-PAGE are indicated on the left. Based on the molecular weight standards for the size exclusion column, fractions 9–11 are >700 kDa; fractions 12–13 are 600–200 kDa; fractions 14–20 are 200–10 kDa. Bottom, western blot analyses of these fractions (9–18) using antibodies against Hsp70/Hsc70, Hsp40, Hsp90a and TCP-1a.