Table 2.
Primer sequences and optimum PCR conditions for amplifying the products
| Gene | Forward (5' to 3') Reverse (5' to 3') |
Product size (bp) | Primer Used (nM) | Annealing Temp (T°) |
| Actb | TCCGCCGCCGGCCCACACT AGTCCTTCTGGCCCATGC |
188 | 0.40 0.40 |
60 |
| Gapdh | CAAGTTCCACGGCACGGTCA CTCGGCACCAGCATCACCC |
118 | 0.40 0.40 |
68 |
| Hprt1 | ACGTCGAGGACTTGGAAAGGGTGTT GGCCTCCCATCTCCTTCATCACATC |
96 | 0.40 0.40 |
68 |
| H2afz | AGAGCCGGCTGCCAGTTCC CAGTCGCGCCCACACGTCC |
85 | 0.20 0.33 |
59 |
| Ubc | GTGACACCATCGAGAAT GACACCTCCCCTCAGAC |
173 | 0.40 0.30 |
60 |
| Ppia | TCCAGGGTTTATGTGCCAGGGTG CGTTTGCCATGGACAAGATGCC |
137 | 0.30 0.30 |
68 |
| Eef1e1 | ACCGCAGAAGAGAAAGCCATAG AGCGATGTAGCCCATAGTAGAGGA |
190 | 0.50 0.50 |
68 |
| G6pdx | AGCCCGCCTCCACTGACTCC ACCACGTTGTCCGCCTGCAC |
88 | 0.40 0.30 |
60 |
| Tbp | GCTGAATATAATCCCAAGCGGTTTGC AAATCAGCGCTGTGGTTCGTGGCTCTC |
73 | 0.30 0.30 |
68 |
| Polr2a | AGACTTCTCGGCCCGCACTG ACTTGGCGCCTGGGTACTGG |
176 | 0.40 0.40 |
68 |
| B2m | GCTCCGTCTTGGGCTTG CGGATGAAACCCAGATACATAG |
152 | 0.33 0.33 |
60 |
| Pgk1 | TGTTGGTCGGGCGAAGCAG CAGTGTCTCCACCGCCGATG |
149 | 0.50 0.20 |
60 |
| Ywhaz | GGTCTGGCCCTTAACTTCTCTGTGTTCTA GCGTGCTGTCTTTGTATGATTCTTCACTT |
142 | 0.50 0.50 |
68 |
| POU5F1 | CGAGTGAGAGGCAACTTGG CGGTTACAGAACCACACACG |
125 | 0.45 0.20 |
57 |