Table 2.
Quantitative analysis of the effects of dnFGFR3 on proliferation and apoptosis during chondrogenesis of Meckel's cartilage and osteogenesis of supra-angular and angular bones.
| Stage of injection | Region of analysis | Time of analysis (days after injection) | H3-p positive (cells/mm2) | TUNEL positive (cells/mm2) | ||
|---|---|---|---|---|---|---|
| Injected | Uninjected | Injected | Uninjected | |||
| HH17/18 | Mandibular mesenchyme | 2 | 308.0 ± 6.8 | 307.0 ± 8.4 | 65.5 ± 7.6 * | 29.7 ± 2.0 |
| HH17/18 | Newly formed Meckel's cartilage | 3 | 143.3 ± 9.3* | 283.7 ± 17.9 | 174.6 ± 10.1* | 41.6 ± 6.7 |
| HH17/18 | Rostral third of Meckel's cartilage | 4 | 287.4 ± 26.3 | 341.7 ± 21.1* | 0 | 0 |
| HH17/18 | Middle third of Meckel's cartilage | 4 | 246.4 ± 18.7 | 221.4 ± 12.1 | 0 | 0 |
| HH17/18 | Caudal third of Meckel's cartilage | 4 | 253.9 ± 19.1 | 249.9 ± 13.2 | 0 | 0 |
| HH17/18 | Osteogenic condensation for supra-angular bone | 4 | 27.3 ± 1.5 | 55.6 ± 1.9 | 24.8 ± 3.9 | 13.9 ± 2.1 |
| HH26 | Differentiated angular bone | 6 | 88.0 ± 7.8* | 115.4 ± 7.2 | 122.2 ± 15.8* | 72 ± 7.6 |
Quantification of H3-p positive cells and TUNEL positive cells was performed as described in Materials and Methods. The areas for quantitative analysis included the entire area of the mandibular mesenchyme (shown in 4A), newly formed cartilage (outlined in Figure 4B), osteogenic condensation for supra-angular bone (outlined in Figure 5A) and differentiated angular bone (outlined in Figures 4 C-F and 5 C-E). All values represent means ± SE of proliferative and apoptotic cells/mm2 in 6−17 sections (described in Materials and Methods) in 4 independent experiments. ND, not determined. Asterisks indicate a statistically significant difference (p<0.01).