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. Author manuscript; available in PMC: 2009 Apr 15.
Published in final edited form as: Dev Biol. 2008 Feb 13;316(2):336–349. doi: 10.1016/j.ydbio.2008.01.035

Table 3.

Quantification of the effects dnFGFR3 on in vitro chondrogenesis of mandibular mesenchyme.

Analysis Days RCAS RCAS-dnFGFR3 RCAS-dnFGFR2
# of aggregates 2 134.3 ± 17.3
(n=6)		 111 ± 5.5
(n=6)		 ND
Size of aggregates (mm2) 2 0.05 ± 0.009
(n=6)		 0.045 ± 0.005
(n=6)		 ND
Relative absorbance 4 117.9 ± 6.3
(n=16)		 73.8 ± 2.0*
(n=12)		 105.8 ± 4.5
(n=12)
6 160.0 ± 6.1
(n=16)		 88.2 ± 8.7*
(n=12)		 146.3 ± 8.2
(n=12)
Relative area stained by Alcian blue 4 8.46 ± 0.33
(n=16)		 5.53 ± 0.14*
(n=12)		 7.87 ± 0.24
(n=12)
6 10.67 ± 0.27
(n=16)		 6.24 ± 0.69*
(n=12)		 10.18 ± 0.67
(n=12)
PNA/Alcian blue 3 1.07 + 0.01
(n=13)		 1.18 + 0.02*
(n=11)		 ND
4 1.12 + 0.02
(n=10)		 1.25 + 0.11*
(n=10)		 ND

The quantification of various parameters was determined as described in the Materials and Methods. Values represent the mean ± S.E. for 6−12 cultures (indicated in parenthesis) in two or three independent experiments. ND, not determined. Asterisks indicate statistical significance at p<0.01.