Fig. 4.

Mutation of the ZEB1 gene leads to ectopic expression of E-cadherin, loss of vimentin and GFAP expression, and decreased proliferation in the ventricular zone of the brain. A. ZEB1 is expressed in the ventricular zone (arrow) of the lateral ventricle. B and C. Expression of vimentin and GFAP, respectively, in the ventricular zone. D. Expression of both vimentin (red) and GFAP (green) is lost in the ventricular zone of ZEB1 gene mutant mice. E. E-cadherin is not expressed in the lateral ventricle in wild-type mice. F-G. E-cadherin becomes ectopically expressed in the ventricular zone of the lateral ventricle in ZEB1 gene null mice. H. E-cadherin is expressed in the third ventricle in ZEB1 gene null mice. I. BrdU incorporation in the ventricular zone of the lateral ventricle. J and K. Double immunolabeling of the boxed region in panel I for BrdU and ZEB1, respectively. L. Overlay of panels J and K. Immunostaining at E15.5 is shown. M. Quantitation of BrdU incorporation into the ventricular zone of the left lateral ventricle (LLV), right lateral ventricle (RLV), hypothalamus (Hypo.), and as a control the tongue. A representative view of areas counted is shown in Supplemental Fig. 3. Bars are 100 μm in panels E, F and I; 50 μm in panels A-D, G and H; 25 μm in J-K.