Fig. 6.

Effect of ZEB1 gene mutation on expression of ZEB2/ SIP1 and Snail mRNA, and TGF-β-dependent induction of vimentin and repression of E-cadherin mRNA. A. Real time PCR was used to assess the effect of ZEB1 gene mutation on expression of other E-box-binding repressors (ZEB2/SIP1, Snail1 or Snail2) in MEFs. Results were normalized to β-actin and GAPDH mRNA, with similar results. B. TGF–β is unable to repress E-cadherin mRNA levels in ZEB1 gene null MEFs. Concentrations of TGF-β are shown. No E-cadherin mRNA was detected in wild-type cells, see Fig. 8B below. C. Basal vimentin mRNA expression is unaffected by ZEB1 gene mutation, but TGF–β induction is lost in ZEB1 gene null MEFs. D. Basal plasminogen activator inhibitor-1 (PAI-1) mRNA is unaffected by ZEB1 gene mutation, and it remains inducible by TGF–β in ZEB1 gene null MEFs.