Figure 2.

(A) Expression of human CD4 by PBMCs of transgenic mice. PBMCs were harvested from the transgenic founder mice, and surface expression of human CD4 was detected by flow cytometry by using a fluorescein isothiocyanate-conjugated mouse monoclonal antibody specifically directed against human CD4. (B) Expression of human CCR5 by PBMCs of transgenic mice. PBMCs harvested from a transgenic mouse derived from transgenic founder mouse 18 and a control mouse were evaluated for surface expression of human CD4 and CCR5 by two-color flow cytometry by using mouse monoclonal antibodies specifically directed against human CD4 and CCR5. The percentage of positive cells in each quadrant is indicated.