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. Author manuscript; available in PMC: 2008 Aug 12.

Published in final edited form as: Biochemistry. 2006 Jul 25;45(29):8988–8999. doi: 10.1021/bi0606602

Table 2.

Steady-State Kinetic Parameters for the α—Keto Acid-Independent Hydrolysis of Acyl-CoA Analogs Catalyzed by M. tuberculosis IPMS^a.

	Without added MgCl₂			With added MgCl₂
	K_m (μM)	k_cat (s⁻¹)	k_cat/K_m (s⁻¹ M⁻¹)	K_m (μM)	k_cat (s⁻¹)	k_cat/K_m (s⁻¹ M⁻¹)
Acetyl-CoA	370 ± 40	(6 ± 0.3) × 10⁻²	150 ± 19	160 ± 29	(3 ± 0.2)× 10⁻²	170 ± 36
Propionyl-CoA	220 ± 40	(4 ± 0.3) × 10⁻²	190 ± 36	ND^b	ND	-
Crotonyl-CoA	50 ± 3	(5 ± 0.2) × 10⁻²	940 ± 72	95 ± 15	(4 ± 0.2)× 10⁻²	400 ± 70

^a

. Performed at 25°C and pH 7.4.

^b

. (ND) Not determined.