Table 4.
Solvent Isotope Effects | |||
---|---|---|---|
Fixed Substrate | Variable Substrate | D2OV | D2OV/K |
Pyruvate | AcCoA | 2.0 ± 0.2 | 1 |
AcCoA | Pyruvate | 1.8 ± 0.2 | 2.2 ± 0.5 |
Solvent isotope effects were determined in 100% H2O or 80 % D2O, at pH 8.3 and 25°C.
Solvent Isotope Effects | |||
---|---|---|---|
Fixed Substrate | Variable Substrate | D2OV | D2OV/K |
Pyruvate | AcCoA | 2.0 ± 0.2 | 1 |
AcCoA | Pyruvate | 1.8 ± 0.2 | 2.2 ± 0.5 |
Solvent isotope effects were determined in 100% H2O or 80 % D2O, at pH 8.3 and 25°C.