Figure 2.

Production and characterization of T cell epitope oligomers. (Left) A size separation of the reaction products of a fragment condensation of the HAS3 4 mer is shown. To identify the products and to control the reaction, titrated amounts of ligase were used [lanes: 1, no ligase; 2, 250 units/ml; 3, 1200 units/ml; M, 100-bp marker (GIBCO)]. The ladder of bands corresponds to oligonucleotides with increasing number of repetitive units (n; indicated on the left side of the gel). The theoretical molecular mass can be calculated by MM = n × 78 bp (one HAS3 unit). Some bands do not match the theoretical MM and probably correspond to cyclic ligation products (e.g., lane 3: ≈480 bp). (Right) An SDS/PAGE separation of the respective polypeptide oligomers is shown. The number of repetitive units is indicated at the bottom of each lane. Their theoretical molecular mass can be calculated by MM = n × 2332 (one HAS3 unit) + 1574 (13 amino acids encoded by the vector).