Figure 5.

Transgenic flies expressing norpA^C1094S display abnormal ERG with slower kinetics. (A) Shown are ERG recordings of norpA^p24, transgenic flies expressing wt norpA, and norpA^C1094S in norpA^p24 or wt genetic background in response to a 2-sec pulse of the orange light. Three independent lines were analyzed, and consistent results were obtained. The kinetics of ERG were examined using the following parameters: latency, defined as time elapsed between stimulation and initiation of ERG (B); initial slope of the response, defined as the rate to reach 50% of the maximal response following the initiation of the response (C); and time to repolarization, time to reach resting potential following the termination of stimulation (D) in wt norpA and norpA^C1094S;norpA^p24. Both latency (B) and time to repolarization (D) are lengthened, and initial slope (C) is reduced in norpA^C1094S;norpA^p24 lines.