Double mutants analysis of norpAC1094S by ERG. norpAC1094S was crossed into inaCp209, InaDp215, and trpp301 mutant backgrounds and analyzed. (A) inaCp209 (Upper), norpAC1094S;inaCp209 (Lower) (B) trpp301 (Upper), norpAC1094S; trpp301 (Lower). (C) InaDp215 (Upper), norpAC1094S;InaDp215 (Lower) (D) Superimposition of ERG from norpAC1094S, norpAC1094S;InaDp215, and norpAC1094S;trpp301. Flies of 1-day posteclosion were subject to heat shock and analyzed. Flies were dark adapted for 3 min prior to the recording using a 2-sec orange light. Multiple animals were recorded and consistent results obtained. Note the difference in the time course and amplitude of the response in trpp301 and norpAC1094S;trpp301.