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. 1997 Dec 23;94(26):14660–14665. doi: 10.1073/pnas.94.26.14660

Figure 1.

Figure 1

Minigenes encoding several CTL epitopes, linked by a spacer of three alanines. The first minigene (rAd-1) encodes the H-2Db-restricted peptide E1A234–243 (26), the H-2Db-restricted peptide HPV16E749–57 (2), the H-2Kb-restricted peptide p53158–166 (49), the H-2Db-restricted peptide E1B192–200 (27), and a Myc tag (31). The second minigene (rAd-2) encodes the HLA-A*0201-restricted CTL epitope HPV16 E786–93 (37), the HLA-A*0201-restricted peptide Flu-matrix58–66 (48), the HLA-A*0201-restricted peptide HPV16 E711–20 (37), the H-2Db-restricted peptide E1B192–200 (27), and a Myc tag (31). Both minigenes are place behind the Ad5 major late promoter (Ad-LMP), which in this configuration is linked to the Ad5 immediate early enhancer, resulting in immediate early expression of the minigenes.