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The Flu-derived epitope encoded by rAd-2 is processed and presented to Flu-specific CTL. HLA-A*0201-positive SAOS cells were infected with rAd and used as stimulator cells in a TNF release assay. SAOS cells infected with rAd-2, but not with rAd-1 or rAd-LAC-Z, activate the Flu-specific CTL clone Q66.9, indicating that the Flu-derived CTL epitope is processed and presented.
