Figure 6.

Vaccination with rAd-1 induces protective immunity against a lethal challenge with Ad5E1A-expressing tumor cells. B6 mice were immunized intraperitoneally with rAd-1 (n = 7), rAd-2 (n = 8), or the Ad5 mutant (Ad5E1A-positive) Ad5ts149 (n = 7) or were injected with PBS/BSA (n = 8) only. Two weeks later, the mice received a subcutaneous challenge of 0.4 × 10⁶ Ad5E1A + Ras cells. Mice immunized with rAd-1 and Ad5ts149 were protected against the outgrowth of Ad5E1A + EJras cells, showing that immunization with rAd induces protective immunity against tumors.