Table 1.

Effect of overexpression of WIP on F-actin content in BJAB cells

Transfection	Cells	Relative F-actin content
Untransfected	BJAB	1.0
pcDNA3	Clone A.11	0.96 ± 0.25
FLAG-WIP	Clone 2	1.40 ± 0.25
	Clone 3	1.79 ± 0.11
	Clone 4	1.55 ± 0.01
FLAG-WIP4	Clone I	1.10 ± 0.17

F-actin content was determined by measuring mean fluorescence intensity in permeabilized cells stained with FITC-phalloidin. The F-actin content represents the mean ratio of the F-actin content in transfected cells to that in untransfected BJAB cell in four experiments. t tests revealed that FLAG-WIP clones 2, 3, and 4 were significantly higher (P ≤ 0.001). There was no significant difference between clone A.11 and clone I (P = 0.20).