Figure 2.

Binding of anti-Galα(1,3)Gal antibodies and human serum to α-galactosidase-transfected PIEC. FACS profiles of stable PIEC cell lines transfected with either human α-galactosidase cDNA or with vector alone were stained with (A) polyclonal chicken anti-laminin antibody to detect Galα(1,3)Gal and with FITC-conjugated goat anti-chicken IgG as control, (B) human natural IgG antibodies and with FITC-conjugated sheep anti-human Ig as control, or (C) NHS and NHS absorbed with Galα(1,3)Gal and with FITC-conjugated sheep anti-human Ig as control.