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Lysis of transfected COS cells by NHS. Pooled NHS was tested for lysis of transfected and nontransfected COS cells in a complement-mediated lysis assay. Titer of NHS on mock-transfected cells and cells transfected with α1,3-galactosyltransferase (GT), α-galactosidase (Galactosidase), and α1,2-fucosyltransferase (HT). Vertical axis, % lysis; horizontal axis, doubling dilutions of human serum commencing at 1/2. Results are representative of three experiments.
