Figure 2.

SDS/PAGE and immunoblotting of proteins and TLC of neutral lipids of the crude extract (lane C), the two-organelle mixture (lane M), the plastid fraction (lane P), and the lipid particle fraction (lane L) from B. napus flower buds. Proteins on the PAGE gel were stained with Coomassie blue (Upper Left) or treated for immunoblotting using antibodies against the 45-kDa oleosin (Upper Right) or the 36-kDa polypeptide (Lower Right). Neutral lipids on the TLC plate were allowed to react with sulfuric acid (Lower Left). In each of the four panels, the same proportional amounts of the four samples were used, with the exception of the crude extract which varied ×0.1 or ×0.5 to reveal their contents clearly. Positions of markers for protein molecular masses and standard lipids are shown on the right. Asterisks indicate oleosins of 48, 45, 35, 25, 23, and 21 kDa, and circles denote the plastid 36-kDa polypeptide.