Figure 2.

DNA gel blot analysis of the transgenic Arabidopsis lines. DNA was extracted from heterozygous plants (+/−) after the third backcross of the indicated lines and from a wild-type Arabidopsis C24 (+/+) used for crosses. For the line SK1-N2, DNA extracted from six albino plants (−/−) segregating in a self-fertilized progeny was analyzed additionally. DNA was digested with PstI or HindIII; fragments were separated by agarose gel, blotted onto a nylon membrane, and hybridized with DNA probes made from cDNA fragments encoded by the respective T-DNA-tagged genes. In DNA from transgenic plants, new hybridizing fragments can be detected in addition to fragments with a wild-type mobility. The albino plants from the progeny of SK1-N2 appear to be homozygous for the T-DNA insertion and completely lack a 2.4-kb fragment.