Table 1.

T-DNA insertion lines for which disrupted genes have been identified

Line	5′ RACE*	i/e†	EST‡	3′ RACE§	DNA blot¶	Homologous genes‖	Probability‖	Putative phenotype**
SK1-13	530	i	—	1,440	+	Arabidopsis DNA-binding	9.5−56	?
						protein POSF21 [Q40088]
SK1-18	1035	e (≈1,130)	Z25619	NT	+	Human ORF [D38555]	2.6−67	?
SK1-N2	179	e (≈570)	—	1,010	+	Antirrhinum majus DAG	7.0−43	Conditional albino
						gene [X95753]
SK1-B2	220	i	—	NT	+	Arabidopsis DNA ligase	2.5−32	Lethal
						[X97924]
SK2-1	466	i	N37338	NT	+	60S acidic ribosomal	2.0−27	?
						protein P2 [O41099]
SK2-2	227	i	N97201	NT	+	?	—	?
SK2-3	202	e (≈724)	—	338	+	?	—	Low transmission  through pollen
SK2-11	132	i	—	NT	NT	60S ribosomal protein  S21E yeast [P05764]	2.9−5	?
SK2-N2	152	i	R30337	NT	+	60S ribosomal protein L31	5.0−8	Narrow leaves
						Nicotiana glutosa [P46290]
SK3-1	246	i	—	1,020	+	Two domains, one peptidyl-	7.2−12	?
						prolyl cis-trans isomerase
						E. coli [P39159] and the
						Synechocystis sp.	2.3−30
						hypothetical protein
						[g1001676]
SK3-3	940	i	N96601	1,150	+	Mice ABC transporter-7	1.8−98	Chlorosis
						[U43892]
SK3-7	190	e (≈1,090)	F13974	900	NT	Aspartyl tRNA synthase	2.0−10	?

^*The lengths of the cDNA sequences, found in a 5′RACE–PCR fragments and not derived from a T-DNA vector are indicated in base pairs. 

^†Cases, in which a new sequence began exactly at a 3′ splice site of the first arp intron were classified as T-DNA integration in introns (i). If a novel sequence began at any other site of the first or second arp exons, 5′ noncoding regions of arp, or at the nptII gene, the T-DNA integration was considered to occur in an exon (e) of the target gene. In such cases it is possible to estimate the extent of the T-DNA deletion (indicated in brackets in base pairs) required for such fusion to happen. 

^‡If sequences of the 5′/3′RACE–PCR products showed a match to known Arabidopsis EST, database accession number of this EST marker is indicated. 

^§For gene identification purposes, or to obtain probes for DNA hybridizations, 3′RACE–PCR was done on the first-strand cDNA. Herein, the total length of sequenced cDNAs are indicated. NT, not tested lines. 

^¶Results of DNA gel blot hybridization were used to confirm T-DNA integration in a gene (+). NT, not tested lines. 

^‖A blast search (NCBI, blast network server) was used to find genes homologous to the T-DNA tagged genes. Genes with highest sequence homology (smallest sum probability is indicated in next column) are listed along with their database accession numbers. For two lines, SK2-2 and SK2-3, no significant homology to known DNA or protein sequences was found, maybe because not sufficient sequence information is available. 

^**Mutant phenotypes associated with gene disruptions were scored in progeny of transgenic lines after third backcross to the wild-type A. thaliana C24. Lines for which no obvious phenotype was found under standard growth conditions are indicated by a question mark.