Figure 3.

p53CP and p53-Ab complex showed differential binding specificity to the p53 binding consensus sequences. Nuclear extract was prepared from mouse H-Tx cells and subjected to gel retardation assay with increasing amounts of nonspecific competitor, poly(dI/dC). All gel shift reactions contained p53 antibody, pAb421 to enhance p53 binding. The oligonucleotides used were WAF, GADD, and T3SF. The positions of p53-Ab complex and p53CP are indicated.