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Mapping the minimal sequence required for p53CP binding. A series of deletion oligonucleotides based on T3SF sequence were synthesized and labeled to define the minimal p53CP binding site. The sequences of these oligonucleotides were listed in Table 1. The gel retardation assay was performed with a poly(dI/dC) concentration of 50 μg/ml. The position of p53CP-DNA complex is indicated.
