Table 1.
Differentiation of ES-cell-derived neural precursors after transplantation into the embryonic rat brain
| Age at analysis | Parenchyma
|
Ventricle
|
|||
|---|---|---|---|---|---|
| Neurons | Glia | NEF | AP | NNT | |
| P0 | TDM | D | + | + | − |
| P0 | TDM | T | − | + | − |
| P0 | TD | − | + | − | − |
| P0 | TM | − | − | + | − |
| P0 | TDM | T | − | ++ | − |
| P0 | D | D | + | + | − |
| P1 | TDM | − | − | ++ | − |
| P1 | TD | − | − | ++ | − |
| P1 | TDM | − | − | + | − |
| P1 | TD | − | − | + | − |
| P1 | TDM | TDM | − | + | − |
| P1 | TDM | − | − | + | − |
| P1 | TDM | D | − | + | − |
| P1 | TDM | TM | − | ++ | − |
| P15 | DM | DM | + | + | + |
| P15 | TDM | TD | − | − | + |
| P15 | TDM | TDM | + | − | + |
| P15 | TM | TM | + | + | + |
| P15 | T | TD | + | − | + |
| P15 | TDM | TM | + | − | + |
| P15 | TD | TD | + | + | + |
ES cells (line J1) aggregated to embryoid bodies and grown for 5–12 days in ITSFn medium were injected into the ventricle of E16–E18 rats. Recipients were sacrificed between P0 and P15 and donor-derived neurons were identified by DNA in situ hybridization in conjunction with immunohistochemical detection of NeuN or by expression of M6 and unequivocal morphological criteria (presence of dendrites and axons). ES-cell-derived astrocytes were detected with an antibody to M2 or by DNA in situ hybridization in conjunction with immunohistochemical detection of GFAP. Intraventricular donor cell clusters were assayed for the presence of nestin-positive neuroepithelial formations (NEF), clusters of undifferentiated, alkaline phosphatase-positive cells (AP; ++, numerous clusters; +, occasional clusters), and differentiated nonneural tissue (NNT). Each row represents one recipient animal. The integration patterns show considerable interindividual variability. There is an increase of glial cells and a decrease of AP-positive cells with increasing survival time. T, telencephalon; D, diencephalon; M, mesencephalon.