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. 1997 Dec 23;94(26):14815–14820. doi: 10.1073/pnas.94.26.14815

Figure 2.

Figure 2

BCNG-1 is a 132 kDa N-glycosylated protein. (A) Western blot analysis of BCNG-1 protein in a mouse brain extract. Ten micrograms of a total brain SDS-extract was loaded per strip then probed with αq1 (1) or αq2 (3) antiserum or αq1 (2) or αq2 (4) antiserum preadsorbed with the GST-d5 fusion protein. The arrow marks the position of native BCNG-1 protein. (B) Western blot using the αq1 antiserum against total brain extract (1), total brain extract pretreated with N-glycosidase F (2), and in vitro translated BCNG-1 protein (3). Positions of molecular mass standards are shown on the left.