Table 1.

Kinetic parameters for PhnO with various acyl-CoAs and aminoalkylphosphonates. A) Values for acyl-CoA’s were determined at a fixed, saturating concentration of (S)-1-aminoethylphosphonic acid. B) Values for aminoalkylphosphonates, and analogs, were determined at a fixed, saturating concentration of AcCoA. NS: not a substrate

Compound	Km (mM)	kcat (s-1)	kcat/Km (M-1 s-1)
A
Acetyl-CoA	0.06 ± 0.01	13.1 ± 0.1	(2.2 ± 0.3) × 105
Propionyl-CoA	0.07 ± 0.01	14.7 ± 0.7	(2.1 ± 0.3) × 105
Malonyl-CoA	0.05 ± 0.01	0.4 ± 0.1	(8.0 ± 0.6) × 103
B
Aminomethyl phosphonic acid	1.7 ± 0.1	7.0 ± 0.1	(4.1 ± 0.2) × 103
2-Aminoethyl phosphonic acid	1.8 ± 0.1	9.0 ± 0.2	(5.0 ± 0.2) × 103
3-Aminopropyl phosphonic acid	4.0 ± 0.4	5.4 ± 0.1	(1.4 ± 0.1) × 103
4-Aminobutylphosphonic acid	13.0 ± 1.0	0.5 ± 0.1	(3.8 ± 0.4) × 101
(S)-1-Aminoethylphosphonic acid	0.17 ± 0.01	13.3 ± 0.1	(7.8 ±0.4) × 104
(R)-1-Aminoethylphosphonic acid	NS
1-Aminopropyl phosphonic acid	4.1 ± 0.2	7.4 ± 0.1	(1.8 ± 0.1) × 103
1-Aminobutyl phosphonic acid	12.5 ± 0.8	1 ± 0.1	(8.0 ± 0.5) × 101
D-Alanine			(3.0 ± 0.3) × 101
2-Aminoethanesulfonic acid	18.9 ± 0.9	14.8 ± 0.2	(7.8 ± 0.3) × 102
2-Aminoethanesulfinic acid	16.4 ± 0.7	20 ± 0.3	(1.2 ± 0.1) × 103