. Author manuscript; available in PMC: 2008 Aug 12.

Published in final edited form as: Biochemistry. 2006 Mar 7;45(9):3033–3039. doi: 10.1021/bi052297p

Table 2.

Kinetic parameters for PhnO with various divalent metal ions. A) Kinetic parameters for S-1AEP were determined at a fixed, saturating concentration of AcCoA and the indicated metal ion. B) Kinetic parameters for divalent metal ion activation at saturating concentrations of S-1AEP and AcCoA

A	K_mS-1AEP (mM)	k_cat (s^-1)	k_cat/k_m (M^-1 s^-1)
Mn²⁺	0.30 ± 0.02	20.6 ± 0.2	(6.9 ± 0.4) × 10⁴
Ni²⁺	0.17 ± 0.01	13.1 ± 0.1	(8.1 ± 0.3) × 10⁴
Co²⁺	0.28 ± 0.06	10.0 ± 0.4	(3.6 ± 0.7) × 10⁴
Mg²⁺	2.00 ± 0.06	17.0 ± 0.3	(8.5 ± 0.3) × 10³
EDTA treated	18.00 ± 0.76	13.0 ± 0.3	(7.2 ± 0.3) × 10²
Non EDTA treated	0.20 ± 0.02	9.2 ± 0.2	(4.6 ± 0.4) × 10⁴
B	K_act (μM)	k_cat (s^-1)	k_cat/K_act (M^-1 s^-1)
Mn²⁺	6.8 ± 0.6	18.7 ± 1.1	(2.7 ± 0.3) × 10⁶
Ni²⁺	0.6 ± 0.1	13.0 ± 0.3	(2.2 ± 0.3) × 10⁷
Co²⁺	1.3 ± 0.4	8.9 ± 1.1	(6.8 ± 2.2) × 10⁶
Cu²⁺	15.9 ± 2.4	4.5 ± 0.2	(2.8 ± 0.4) × 10⁵
Mg²⁺	850 ± 63	16.8 ± 0.2	(2.0 ± 0.1) × 10⁴