Figure 2.

Calyculin A inhibits forskolin- but not PMA-mediated potentiation of striatal NMDA responses. The NMDA-induced currents of striatal mRNA-injected oocytes were measured before and 8 min after 1-min application of 50 μM forskolin (A) or before and 12 min after 1-min application of 10 nM PMA (C). (B and D) The same protocol was followed after injecting oocytes with calyculin A to a final intracellular concentration of 500 nM 60–90 min before voltage-clamping. The intracellular concentration of calyculin A was calculated by assuming standard oocytes with a volume of 0.5 μl.