Figure 3.
Western blot detection of syntaxin from rat brain and squid optic lobe homogenates. Two electrophoresis procedures were performed. Syntaxin was detected with three different antibodies: HPC1, monoclonal antibody raised against rat syntaxin 1; CA and CB, crude antisera from rabbits immunized with the rat cDNA fusion proteins (His)6-syntaxin 1A and (His)6-syntaxin 1B, respectively. Total amount of protein loaded was 5 μg for rat brain homogenates and 30 μg for squid optic lobe homogenates.