PCR amplification of ρ+ and ρo DNA and of DNA from the supernatant (S) and the pelleted cell debris (P) after 10 min of boiling of the buffy coat of an AD patient (subject 1) and a control (subject 2), using primers corresponding to the three COX genes. The predicted sizes of the PCR products, in bp, are at right. M, DNA Mass Ladder markers (GIBCO/BRL) with sizes, in bp, at left.