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. 1997 Dec 23;94(26):14900–14905. doi: 10.1073/pnas.94.26.14900

Figure 2.

Figure 2

Detection of the five missense mutations in CO1 and CO2 genes amplified from DNA of 143B-TK− and 143B-87 ρ° cells by restriction endonuclease digestion. PCR products obtained from 143B-TK− DNA are shown in lanes 3 and 5 for CO1 and lanes 7, 9, and 11 for CO2. PCR products obtained from 143B-87 ρ° DNA are shown in lanes 4 and 6 for CO1 and lanes 8, 10, and 12 for CO2. Detection of the np 6366 G-to-A mutation in CO1 by BsmAI digestion is presented in lanes 3 and 4. Detection of the np 7146 A-to-G mutation in CO1 by NruI digestion is shown in lanes 5 and 6. The double band in lane 6 is most likely the result of partial digestion. Detection of the np 7650 C-to-T mutation by HphI digestion is shown in lanes 7 and 8. Detection of the np 7868 C-to-T mutation by MseI digestion is shown in lanes 9 and 10. Detection of the np 8021 A-to-G mutations by HpaII digestion is shown in lanes 11 and 12. Lanes 1 and 2 are size standards: lane 1 is a ∅X174 HaeIII digest and lane 2 is a 1-kb DNA ladder.