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Effect of loperamide (L; 30 μM) on levels of intracellular calcium after release of intracellular calcium and subsequent activation of SOC channels in HL-60 cells. Elevation of intracellular calcium levels was by (A) 10 μM N-formyl-met-leu-phe (FMLP), (B) 10 μM histamine (H), and (C) 10 μM sphingosine (S). (D) Effect of loperamide (L) on levels of intracellular calcium in low-calcium buffer followed by reintroduction of 2 mM Ca2+. Cells were loaded with fluo3-AM.
