Figure 3.
Effect of loperamide (L; 30 μM) on levels of intracellular calcium after release of intracellular calcium and subsequent activation of SOC channels in (A) RBL-2H3 cells, (B) NIH 3T3 cells, (C) DDT-MF2 cells, (D) GH4C1 cells, and (E) astrocytoma 1321N cells. Intracellular calcium levels were elevated by using (A) 10 μM N-ethylcarboxamidoadenosine (NECA) + 20 μg/ml dinitrophenol conjugated with human serum albumin, (B and C) 15 nM thapsigargin (T), (D) 1 μM ionomycin (I), and (E) 10 μM ATP. (F) Effect of loperamide added before ATP in astrocytoma 1321N cells. Cells were loaded with fura-2AM.