Table 1.
Gluconeogenic enzyme activities and glycogen stores in starved and fed 11 β-HDS-1 −/− mutants and 11 β-HSD-1 +/+ wild-type controls
| 11β-HSD-1 −/−
|
11β-HSD-1 +/+
|
|||
|---|---|---|---|---|
| Fed (n = 8) | Starved (n = 8) | Fed (n = 8) | Starved (n = 8) | |
| G6Pase, intact microsomes | ||||
| Vmax (μmol/min·mg) | 0.42 ± 0.04 | 0.42 ± 0.05* | 0.43 ± 0.07 | 0.63 ± 0.04† |
| KM (mM) | 5.26 ± 0.42 | 5.93 ± 0.57* | 6.06 ± 0.58 | 7.86 ± 0.77† |
| G6Pase, disrupted microsomes | ||||
| Vmax (μmol/min·mg) | 0.54 ± 0.03 | 0.64 ± 0.06 | 0.53 ± 0.02 | 0.81 ± 0.07† |
| KM (mM) | 0.40 ± 0.06 | 0.44 ± 0.09 | 0.56 ± 0.13 | 0.50 ± 0.10 |
| PEPCK | ||||
| Vmax (nmol/min·mg) | 3.5 ± 0.5 | 11.3 ± 1.4*† | 5.3 ± 0.5 | 15.7 ± 1.7† |
| Glucokinase | ||||
| Vmax (nmol/min·mg) | 28.4 ± 3.0 | 19.6 ± 1.8† | 29.4 ± 4.3 | 22.8 ± 2.6 |
| Glycogen μg/mg protein | 528 ± 99* | 77.5 ± 8.1† | 302 ± 37.9 | 73.8 ± 8.2† |
| Animal weight grams | 30.2 ± 1.2 | 25.9 ± 1.1† | 27.1 ± 2.0 | 23.3 ± 0.9 |
G6Pase and glycogen are per mg microsomal protein. PEPCK and glucokinase are per mg cytosolic protein.
*P < 0.05 compared with corresponding 11β-HSD-1 +/+ value.
†
P < 0.05 compared with corresponding fed value.