Figure 3.

Time-sequential changes of transcripts for SIG genes in dark-adapted plants after exposure to light. (A) A. thaliana grown for 3 weeks was incubated in the dark for 3 days or maintained under the same light conditions for plants indicated as “mature.” Leaves of individual dark-adapted plants were harvested at 0, 3, 7, 15, and 24 hr after initiation of the exposure to light at 3,000 lux. RT-PCR products were subjected to electrophoresis after PCR cycles when intensities of signals for ACT2 were unsaturated and comparable among RNA fractions harvested at different illumination times. RT-PCR products (5 μl from 50 μl of reaction mixture) were electrophoresed in 3% agarose gel (Agarose H, NipponGene), stained with SYBR green I (FMC BioProducts), and observed by FluorImager SI (Molecular Dynamics). (B) RT-PCR products of 0- and 3-hr illumination after indicated PCR cycles were electrophoresed and stained with SYBR green I.